SIRF: A Single-cell Assay for in situ Protein Interaction with Nascent DNA Replication Forks
The duplication of DNA is a basic course of that’s required for the switch of the genetic data from mum or dad to daughter cells. Aberrant DNA replication course ofes are related with various illness phenotypes, together with developmental defects, ageing issues, blood issues comparable to Fanconi Anemia, elevated irritation and most cancers. Therefore, the event of instruments to check proteins related with error-free DNA replication course ofes is of paramount significance. So far, strategies to check proteins related with nascent replication forks relied on typical immunofluorescence and immunoprecipitation assays of 5′-ethylene-2′-deoxyuridine (EdU) labeled DNA (iPOND).
While significantly informative and vital, these strategies lack specificities for nascent fork interactions (e.g., IF) or assay a median change of tens of millions of cells with out single-cell decision (e.g., iPOND). The assay system described right here combines proximity ligation assay (PLA) with EdU coupled click-iT chemistry, which we termed “in situ protein interplay with nascent DNA replication forks (SIRF)”. This technique allows delicate and quantitative evaluation of protein interactions with nascent DNA replication forks with single-cell decision, and may additional be paired with typical immunofluorescence marker evaluation for added multi-parameter evaluation. New insights have been offered into each the atmospheric chemistry of isoprene and the picturechemistry of TiO2 nanoparticles.
Titania (TiO2) nanoparticles are lively photocatalysts, and isoprene (C5H8) is a biogenic risky natural compound that contributes crucially to international particulate matter era. Herein, the direct photooxidation of isoprene by titanium oxide cluster anions with dimensions as much as a nanosize by each ultraviolet (UV) and visual (Vis) gentle excitations has been efficiently recognized via mass spectrometric experiments mixed with quantum chemistry calculations. The potential position of “dry” titania in atmospheric isoprene oxidation has been revealed, and a transparent image of the photooxidation mechanism on titanium oxide nanoparticles has been offered explicitly on the molecular stage.
The adsorption of isoprene on the atomic oxygen radicals (O•-) of titanium oxide clusters results in the formation of the essential interfacial state (IS) inside the band hole of titanium oxides. This IS is demonstrated to be the numerous issue in delivering the electron from the π orbital of C5H8 to the Ti3d orbital in the photooxidation course of and creating photoactivity in the Vis area. It is revealed that after the photogeneration of the O•- radicals by UV excitation on the TiO2 particle floor, the next reactions will be induced by Vis excitation via the IS. This multicolor technique in each the UV and Vis areas can improve the effectivity of photo voltaic vitality harvesting and enhance the product yield of the photocatalysis on TiO2 nanoparticles.
Computational Analysis Reveals a Critical Point Mutation in the N-Terminal Region of the Signaling Lymphocytic Activation Molecule Responsible for the Cross-Species Infection with Canine Distemper Virus
Infection of hosts by morbilliviruses is facilitated by the interplay between viral hemagglutinin (H-protein) and the signaling lymphocytic activation molecule (SLAM). Recently, the purposeful significance of the n-terminal area of human SLAM as a measles virus receptor was demonstrated. However, the purposeful roles of this area in the an infection course of by different morbilliviruses and host vary willpower stay unknown, partly as a result of this area is extremely versatile, which has hampered correct construction willpower of this area by X-ray crystallography.
In this examine, we analyzed the interplay between the H-protein from canine distemper virus (CDV-H) and SLAMs by a computational chemistry method. Molecular dynamics simulations and fragment molecular orbital evaluation demonstrated that the distinctive His28 in the N-terminal area of SLAM from Macaca is a key determinant that allows the formation of a secure interplay with CDV-H, offering a foundation for CDV an infection in Macaca. The computational chemistry method offered ought to allow the willpower of molecular interactions involving areas of proteins which can be tough to foretell from crystal buildings due to their excessive flexibility.
Reaction Mechanisms and Rate Constants of Auto-Catalytic Urethane Formation and Cleavage Reactions
Cefuroxime Sodium |
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C023-1G | TOKU-E | 1 g | EUR 145.2 |
Cefuroxime Sodium |
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C023-5G | TOKU-E | 5 g | EUR 402 |
Cefuroxime (sodium) |
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HY-B1256 | MedChemExpress | 1g | EUR 192 |
Cefuroxime Sodium |
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317455 | MedKoo Biosciences | 100.0mg | EUR 120 |
Cefuroxime sodium |
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T1224-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Cefuroxime sodium |
Cefuroxime sodium |
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Description: Cefuroxime sodium |
Cefuroxime sodium |
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T1224-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Cefuroxime sodium |
Cefuroxime sodium |
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Description: Cefuroxime sodium |
Cefuroxime sodium |
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T1224-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Cefuroxime sodium |
Cefuroxime |
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GA9610-100MG | Glentham Life Sciences | 100 mg | EUR 84 |
Cefuroxime |
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GA9610-1G | Glentham Life Sciences | 1 g | EUR 187.2 |
Cefuroxime |
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GA9610-250MG | Glentham Life Sciences | 250 mg | EUR 112.8 |
Cefuroxime |
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GA9610-1 | Glentham Life Sciences | 1 | EUR 125.1 |
Cefuroxime |
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GA9610-100 | Glentham Life Sciences | 100 | EUR 39.4 |
Cefuroxime |
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GA9610-250 | Glentham Life Sciences | 250 | EUR 63.3 |
Cefuroxime axetil |
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584893 | MedKoo Biosciences | 350.0mg | EUR 535 |
Cefuroxime axetil |
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C6478-25 | ApexBio | 25mg | EUR 56 |
Cefuroxime axetil |
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T1227-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Cefuroxime axetil |
Cefuroxime axetil |
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Description: Cefuroxime axetil |
Cefuroxime axetil |
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T1227-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Cefuroxime axetil |
Cefuroxime axetil |
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T1227-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Cefuroxime axetil |
Cefuroxime axetil |
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T1227-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Cefuroxime axetil |
Cefuroxime (free base) |
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412546 | MedKoo Biosciences | 100.0mg | EUR 418 |
Cefuroxime free acid |
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C282-1G | TOKU-E | 1 g | EUR 79.57 |
Description: C16H16N4O8S |
Cefuroxime free acid |
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C282-5G | TOKU-E | 5 g | EUR 264.35 |
Description: C16H16N4O8S |
Descarbamoyl cefuroxime |
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T11000-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Descarbamoyl cefuroxime |
Descarbamoyl cefuroxime |
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T11000-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Descarbamoyl cefuroxime |
Descarbamoyl cefuroxime |
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T11000-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Descarbamoyl cefuroxime |
Descarbamoyl cefuroxime |
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T11000-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Descarbamoyl cefuroxime |
Descarbamoyl cefuroxime |
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T11000-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Descarbamoyl cefuroxime |
Cefuroxime Axetil (USP grade powder) |
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51R-U098220 | Fitzgerald | 350 mg | EUR 603.6 |
Description: Cefuroxime Axetil (USP grade powder) chemical reference substance |
Cefuroxime Axetil d-3 iso (USP grade powder) |
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51R-U098231 | Fitzgerald | 10 mg | EUR 1975.2 |
Description: Cefuroxime Axetil d-3 iso (USP grade powder) chemical reference substance |
Cefazolin Sodium Salt |
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B1530-1G | Biovision | each | EUR 170.4 |
Cefazolin Sodium Salt |
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B1530-5G | Biovision | each | EUR 430.8 |
Cefazolin (sodium salt) |
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C4183-1000 | ApexBio | 1 g | EUR 201.6 |
Description: Cefazolin is a semisynthetic antibiotic with a broad spectrum of antibacterial activity. Cefazolin has exhibited high activity against Gram-positive bacteria and Gram-negative bacteria [1]. |
Cefazolin (sodium salt) |
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C4183-250 | ApexBio | 250 mg | EUR 126 |
Description: Cefazolin is a semisynthetic antibiotic with a broad spectrum of antibacterial activity. Cefazolin has exhibited high activity against Gram-positive bacteria and Gram-negative bacteria [1]. |
Cefazolin (sodium salt) |
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C4183-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 135.6 |
Description: Cefazolin is a semisynthetic antibiotic with a broad spectrum of antibacterial activity. Cefazolin has exhibited high activity against Gram-positive bacteria and Gram-negative bacteria [1]. |
Cefazolin (sodium salt) |
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C4183-500 | ApexBio | 500 mg | EUR 165.6 |
Description: Cefazolin is a semisynthetic antibiotic with a broad spectrum of antibacterial activity. Cefazolin has exhibited high activity against Gram-positive bacteria and Gram-negative bacteria [1]. |
Cefoxitin (sodium salt) |
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C4849-1000 | ApexBio | 1 g | EUR 289.2 |
Description: Cefoxitin (sodium salt) is a second generation cephalosporin antibiotic that was used to treat a wide range of gram-negative and gram-positive bacteria [1][2]. |
Cefoxitin (sodium salt) |
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C4849-250 | ApexBio | 250 mg | EUR 135.6 |
Description: Cefoxitin (sodium salt) is a second generation cephalosporin antibiotic that was used to treat a wide range of gram-negative and gram-positive bacteria [1][2]. |
Cefoxitin (sodium salt) |
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C4849-500 | ApexBio | 500 mg | EUR 196.8 |
Description: Cefoxitin (sodium salt) is a second generation cephalosporin antibiotic that was used to treat a wide range of gram-negative and gram-positive bacteria [1][2]. |
Cefonicid (sodium salt) |
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C3812-1000 | ApexBio | 1 g | EUR 487.2 |
Description: Cefonicid is a second-generation cephalosporin antibiotic.Cephalosporins, the largest and most diverse family of antibiotics of the beta-lactam group, are structurally and pharmacologically related to the penicillins. |
Cefonicid (sodium salt) |
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C3812-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 135.6 |
Description: Cefonicid is a second-generation cephalosporin antibiotic.Cephalosporins, the largest and most diverse family of antibiotics of the beta-lactam group, are structurally and pharmacologically related to the penicillins. |
Cefonicid (sodium salt) |
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C3812-500 | ApexBio | 500 mg | EUR 301.2 |
Description: Cefonicid is a second-generation cephalosporin antibiotic.Cephalosporins, the largest and most diverse family of antibiotics of the beta-lactam group, are structurally and pharmacologically related to the penicillins. |
Cefoxitin Sodium Salt |
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B1892-1G | Biovision | each | EUR 222 |
Cefoxitin Sodium Salt |
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B1892-500 | Biovision | each | EUR 157.2 |
Cefoxitin Sodium Salt |
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B1892-5G | Biovision | each | EUR 692.4 |
Cefoxitin sodium salt |
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GA4412-1G | Glentham Life Sciences | 1 g | EUR 132 |
Cefoxitin sodium salt |
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GA4412-250MG | Glentham Life Sciences | 250 mg | EUR 74.4 |
Cefoxitin sodium salt |
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GA4412-5G | Glentham Life Sciences | 5 g | EUR 351.6 |
Cefazolin sodium salt |
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GA4751-1G | Glentham Life Sciences | 1 g | EUR 103.2 |
Cefazolin sodium salt |
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GA4751-5G | Glentham Life Sciences | 5 g | EUR 208.8 |
Ceftiofur sodium salt |
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GA9758-1G | Glentham Life Sciences | 1 g | EUR 132 |
Cefapirin sodium salt |
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GP5306-1G | Glentham Life Sciences | 1 g | EUR 189.6 |
Cefapirin sodium salt |
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GP5306-250MG | Glentham Life Sciences | 250 mg | EUR 103.2 |
Ceftiofur sodium salt |
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GA9758-1 | Glentham Life Sciences | 1 | EUR 67.9 |
Cefoxitin sodium salt |
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GA4412-1 | Glentham Life Sciences | 1 | EUR 79.1 |
Cefoxitin sodium salt |
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GA4412-250 | Glentham Life Sciences | 250 | EUR 31.7 |
Cefoxitin sodium salt |
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GA4412-5 | Glentham Life Sciences | 5 | EUR 261 |
Cefazolin sodium salt |
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GA4751-1 | Glentham Life Sciences | 1 | EUR 55.4 |
Cefazolin sodium salt |
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GA4751-5 | Glentham Life Sciences | 5 | EUR 142.4 |
Cefapirin sodium salt |
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GP5306-1 | Glentham Life Sciences | 1 | EUR 126.5 |
Cefapirin sodium salt |
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GP5306-250 | Glentham Life Sciences | 250 | EUR 55.4 |
Cefotaxime Sodium Salt |
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abx188641-100g | Abbexa | 100 g | EUR 610.8 |
Cefsulodin (sodium salt) |
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C5593-1000 | ApexBio | 1 g | EUR 268.8 |
Description: Cefsulodin, formerly named as SCE-129, is a cephalosporin with a spectrum of antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, and most other gram-positive cocci [1]. |
Cefsulodin (sodium salt) |
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C5593-250 | ApexBio | 250 mg | EUR 147.6 |
Description: Cefsulodin, formerly named as SCE-129, is a cephalosporin with a spectrum of antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, and most other gram-positive cocci [1]. |
Cefsulodin (sodium salt) |
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C5593-500 | ApexBio | 500 mg | EUR 216 |
Description: Cefsulodin, formerly named as SCE-129, is a cephalosporin with a spectrum of antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, and most other gram-positive cocci [1]. |
Cefotaxime (sodium salt) |
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C4588-1000 | ApexBio | 1 g | EUR 151.2 |
Description: MIC: <0.1 ?g/ml for S. pneumoniae Cefotaxime is a cephalosporin antibiotic. |
Cefotaxime (sodium salt) |
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C4588-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 135.6 |
Description: MIC: <0.1 ?g/ml for S. pneumoniae Cefotaxime is a cephalosporin antibiotic. |
Cefotaxime (sodium salt) |
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C4588-500 | ApexBio | 500 mg | EUR 129.6 |
Description: MIC: <0.1 ?g/ml for S. pneumoniae Cefotaxime is a cephalosporin antibiotic. |
Cefotaxime (sodium salt) |
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C4588-5000 | ApexBio | 5 g | EUR 181.2 |
Description: MIC: <0.1 ?g/ml for S. pneumoniae Cefotaxime is a cephalosporin antibiotic. |
Cefotaxime Sodium Salt |
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20-abx082178 | Abbexa |
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Cefotaxime sodium salt |
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GA3583-1G | Glentham Life Sciences | 1 g | EUR 132 |
Cefotaxime sodium salt |
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GA3583-25G | Glentham Life Sciences | 25 g | EUR 600 |
Cefotaxime sodium salt |
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GA3583-5G | Glentham Life Sciences | 5 g | EUR 217.2 |
Cefditoren sodium salt |
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GA4627-1G | Glentham Life Sciences | 1 g | EUR 753.6 |
Cefotaxime (sodium salt) |
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HY-A0088 | MedChemExpress | 10mM/1mL | EUR 135.6 |
Cefotaxime sodium salt |
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GA3583-1 | Glentham Life Sciences | 1 | EUR 60.3 |
Cefotaxime sodium salt |
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GA3583-25 | Glentham Life Sciences | 25 | EUR 372.8 |
Cefotaxime sodium salt |
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GA3583-5 | Glentham Life Sciences | 5 | EUR 135.6 |
Cefditoren sodium salt |
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GA4627-1 | Glentham Life Sciences | 1 | EUR 593.3 |
Cefotaxime Sodium Salt |
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40310008-1 | Bio-WORLD | 5 g | EUR 89.68 |
Cefotaxime Sodium Salt |
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40310008-2 | Bio-WORLD | 10 g | EUR 134.51 |
Cefotaxime Sodium Salt |
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40310008-3 | Bio-WORLD | 25 g | EUR 187.43 |
Cefotaxime Sodium Salt |
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40310008-4 | Bio-WORLD | 50 g | EUR 272.69 |
Cefotaxime Sodium Salt |
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40310008-5 | Bio-WORLD | 100 g | EUR 455.6 |
Cefsulodin Sodium Salt |
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40310029-1 | Bio-WORLD | 100 mg | EUR 34.32 |
Cefsulodin Sodium Salt |
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40310029-2 | Bio-WORLD | 1 g | EUR 89.68 |
Cefamandole sodium salt |
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C079-100MG | TOKU-E | 100 mg | EUR 177.6 |
Cefamandole sodium salt |
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C079-500MG | TOKU-E | 500 mg | EUR 568.8 |
Cefamandole (sodium salt) |
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C3541-100 | ApexBio | 100 mg | EUR 292.8 |
Description: MIC: 0.25-2 mg/L for different E. coli strainsCefamandole is a cephalosporin antibiotic.The cephalosporins are a class of ?-lactam antibiotics originally derived from the fungus Acremonium, which was previously known as "Cephalosporium". |
Cefamandole (sodium salt) |
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C3541-50 | ApexBio | 50 mg | EUR 182.4 |
Description: MIC: 0.25-2 mg/L for different E. coli strainsCefamandole is a cephalosporin antibiotic.The cephalosporins are a class of ?-lactam antibiotics originally derived from the fungus Acremonium, which was previously known as "Cephalosporium". |
Cefamandole (sodium salt) |
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C3541-500 | ApexBio | 500 mg | EUR 710.4 |
Description: MIC: 0.25-2 mg/L for different E. coli strainsCefamandole is a cephalosporin antibiotic.The cephalosporins are a class of ?-lactam antibiotics originally derived from the fungus Acremonium, which was previously known as "Cephalosporium". |
Ceftizoxime sodium salt |
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GA1247-1G | Glentham Life Sciences | 1 g | EUR 98.4 |
Ceftizoxime sodium salt |
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GA1247-250MG | Glentham Life Sciences | 250 mg | EUR 70.8 |
Cefpodoxime sodium salt |
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GA7534-100MG | Glentham Life Sciences | 100 mg | EUR 648 |
Ceftriaxone (sodium salt) |
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HY-B0712B | MedChemExpress | 500mg | EUR 207.6 |
Cefamandole sodium salt |
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GA9567-100 | Glentham Life Sciences | 100 | EUR 75.3 |
Cefamandole sodium salt |
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GA9567-500 | Glentham Life Sciences | 500 | EUR 248.6 |
Ceftizoxime sodium salt |
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GA1247-1 | Glentham Life Sciences | 1 | EUR 51.4 |
Ceftizoxime sodium salt |
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GA1247-250 | Glentham Life Sciences | 250 | EUR 28.4 |
Cefmetazole sodium salt |
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GA4116-1 | Glentham Life Sciences | 1 | EUR 113.1 |
Cefmetazole sodium salt |
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GA4116-250 | Glentham Life Sciences | 250 | EUR 37.7 |
Cefpodoxime sodium salt |
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GA7534-100 | Glentham Life Sciences | 100 | EUR 506.2 |
Ceftriaxone sodium salt |
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T10754-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Ceftriaxone sodium salt |
Ceftriaxone sodium salt |
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T10754-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Ceftriaxone sodium salt |
Ceftriaxone sodium salt |
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T10754-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Ceftriaxone sodium salt |
Ceftriaxone sodium salt |
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T10754-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: Ceftriaxone sodium salt |
Ceftriaxone sodium salt |
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T10754-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: Ceftriaxone sodium salt |
Cefoperazone (sodium salt) |
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C3913-10000 | ApexBio | 10 g | EUR 483.6 |
Description: Cefoperazone is a new semisynthetic cephalosporin with a broad spectrum of antibacterial activity. Cefoperazone shows high activity against gram-positive bacteria and gram-negative bacilli, such as Escherichia coli, Klebsiella pneumoniae, and Proteus species [1]. |
Cefoperazone (sodium salt) |
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C3913-5000 | ApexBio | 5 g | EUR 320.4 |
Description: Cefoperazone is a new semisynthetic cephalosporin with a broad spectrum of antibacterial activity. Cefoperazone shows high activity against gram-positive bacteria and gram-negative bacilli, such as Escherichia coli, Klebsiella pneumoniae, and Proteus species [1]. |
Cefoperazone sodium salt |
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GA8838-1G | Glentham Life Sciences | 1 g | EUR 69.6 |
Cefoperazone sodium salt |
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GA8838-25G | Glentham Life Sciences | 25 g | EUR 246 |
Cefoperazone sodium salt |
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GA8838-5G | Glentham Life Sciences | 5 g | EUR 122.4 |
Cefoperazone (sodium salt) |
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HY-B0210A | MedChemExpress | 500mg | EUR 129.6 |
Cefoperazone sodium salt |
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GA8838-1 | Glentham Life Sciences | 1 | EUR 27.8 |
Cefoperazone sodium salt |
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GA8838-25 | Glentham Life Sciences | 25 | EUR 174 |
Cefoperazone sodium salt |
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GA8838-5 | Glentham Life Sciences | 5 | EUR 71.1 |
Cefoperazone sodium salt |
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T1218-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: Cefoperazone sodium salt |
Cefoperazone sodium salt |
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T1218-1g | TargetMol Chemicals | 1g | Ask for price |
Description: Cefoperazone sodium salt |
Cefoperazone sodium salt |
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T1218-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: Cefoperazone sodium salt |