SIRF: A Single-cell Assay for in situ Protein Interaction with Nascent DNA Replication Forks
The duplication of DNA is a basic course of that’s required for the switch of the genetic data from mum or dad to daughter cells. Aberrant DNA replication course ofes are related with various illness phenotypes, together with developmental defects, ageing issues, blood issues comparable to Fanconi Anemia, elevated irritation and most cancers. Therefore, the event of instruments to check proteins related with error-free DNA replication course ofes is of paramount significance. So far, strategies to check proteins related with nascent replication forks relied on typical immunofluorescence and immunoprecipitation assays of 5′-ethylene-2′-deoxyuridine (EdU) labeled DNA (iPOND).
While significantly informative and vital, these strategies lack specificities for nascent fork interactions (e.g., IF) or assay a median change of tens of millions of cells with out single-cell decision (e.g., iPOND). The assay system described right here combines proximity ligation assay (PLA) with EdU coupled click-iT chemistry, which we termed “in situ protein interplay with nascent DNA replication forks (SIRF)”. This technique allows delicate and quantitative evaluation of protein interactions with nascent DNA replication forks with single-cell decision, and may additional be paired with typical immunofluorescence marker evaluation for added multi-parameter evaluation. New insights have been offered into each the atmospheric chemistry of isoprene and the picturechemistry of TiO2 nanoparticles.
Titania (TiO2) nanoparticles are lively photocatalysts, and isoprene (C5H8) is a biogenic risky natural compound that contributes crucially to international particulate matter era. Herein, the direct photooxidation of isoprene by titanium oxide cluster anions with dimensions as much as a nanosize by each ultraviolet (UV) and visual (Vis) gentle excitations has been efficiently recognized via mass spectrometric experiments mixed with quantum chemistry calculations. The potential position of “dry” titania in atmospheric isoprene oxidation has been revealed, and a transparent image of the photooxidation mechanism on titanium oxide nanoparticles has been offered explicitly on the molecular stage.
The adsorption of isoprene on the atomic oxygen radicals (O•-) of titanium oxide clusters results in the formation of the essential interfacial state (IS) inside the band hole of titanium oxides. This IS is demonstrated to be the numerous issue in delivering the electron from the π orbital of C5H8 to the Ti3d orbital in the photooxidation course of and creating photoactivity in the Vis area. It is revealed that after the photogeneration of the O•- radicals by UV excitation on the TiO2 particle floor, the next reactions will be induced by Vis excitation via the IS. This multicolor technique in each the UV and Vis areas can improve the effectivity of photo voltaic vitality harvesting and enhance the product yield of the photocatalysis on TiO2 nanoparticles.
Computational Analysis Reveals a Critical Point Mutation in the N-Terminal Region of the Signaling Lymphocytic Activation Molecule Responsible for the Cross-Species Infection with Canine Distemper Virus
Infection of hosts by morbilliviruses is facilitated by the interplay between viral hemagglutinin (H-protein) and the signaling lymphocytic activation molecule (SLAM). Recently, the purposeful significance of the n-terminal area of human SLAM as a measles virus receptor was demonstrated. However, the purposeful roles of this area in the an infection course of by different morbilliviruses and host vary willpower stay unknown, partly as a result of this area is extremely versatile, which has hampered correct construction willpower of this area by X-ray crystallography.
In this examine, we analyzed the interplay between the H-protein from canine distemper virus (CDV-H) and SLAMs by a computational chemistry method. Molecular dynamics simulations and fragment molecular orbital evaluation demonstrated that the distinctive His28 in the N-terminal area of SLAM from Macaca is a key determinant that allows the formation of a secure interplay with CDV-H, offering a foundation for CDV an infection in Macaca. The computational chemistry method offered ought to allow the willpower of molecular interactions involving areas of proteins which can be tough to foretell from crystal buildings due to their excessive flexibility.
Reaction Mechanisms and Rate Constants of Auto-Catalytic Urethane Formation and Cleavage Reactions
Cefuroxime sodium salt |
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GA1625-5 | Glentham Life Sciences | 5 | EUR 208 |
Cefuroxime sodium salt |
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GA1625-5G | Glentham Life Sciences | 5 g | EUR 288 |
Cefuroxime Sodium Salt, 86-100% |
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84755 | Sisco Laboratories | 2 Gms | EUR 9.38 |
Description: Part B |
Oleic acid, For biochemistry |
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RM5371-5ML | EWC Diagnostics | 1 unit | EUR 50.98 |
Description: Oleic acid, For biochemistry |
Amino acid-kit ?(for biochemistry) |
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K007-1KT | EWC Diagnostics | 1 unit | EUR 67.59 |
Description: Amino acid-kit ?(for biochemistry) |
Amino acid-kit ?(for biochemistry) |
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K007-5KT | EWC Diagnostics | 1 unit | EUR 303.95 |
Description: Amino acid-kit ?(for biochemistry) |
Cefuroxime Sodium |
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317455 | MedKoo Biosciences | 100.0mg | EUR 120 |
Cefuroxime Sodium |
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C023-1G | TOKU-E | 1 g | EUR 77.89 |
Description: C16H15N4N/AO8S |
Cefuroxime Sodium |
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C023-5G | TOKU-E | 5 g | EUR 258.61 |
Description: C16H15N4N/AO8S |
Cefuroxime (sodium) |
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HY-B1256 | MedChemExpress | 1g | EUR 192 |
Cefuroxime sodium |
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Cefuroxime sodium |
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Cefuroxime sodium |
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Cefuroxime sodium |
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Cefuroxime sodium |
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Cefuroxime sodium |
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MBS577332-100mg | MyBiosource | 100mg | EUR 130 |
Cefuroxime sodium |
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Cefuroxime sodium |
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Cefuroxime sodium |
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Cefuroxime sodium |
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MBS3841671-100mg | MyBiosource | 100mg | EUR 170 |
Cefuroxime sodium |
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Cefuroxime sodium |
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Cefuroxime sodium |
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MBS3605045-100mg | MyBiosource | 100mg | EUR 210 |
Cefuroxime sodium |
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MBS3605045-50mg | MyBiosource | 50mg | EUR 200 |
Cefuroxime sodium |
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MBS3605045-5x100mg | MyBiosource | 5x100mg | EUR 630 |
Acetobromo-D-Glucose extrapure for biochemistry, 98% |
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34437 | Sisco Laboratories | 10 Gms | EUR 55.13 |
Description: Part B |
L-Lysine Monohydrate (base) extrapure for biochemistry, 99% |
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85187 | Sisco Laboratories | 25 Gms | EUR 13.48 |
Description: Part A |
D-Maltose Monohydrate for bacteriology and biochemistry |
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59338 | Sisco Laboratories | 100 Gms | EUR 1.85 |
Description: Part A |
Cefuroxime Sodium Salt ((6R, 7R)-3-[[(Aminocarbonyl)oxy]methyl]-7-[[(2Z)-2-furanyl(methoxyimino)acetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid Sodium Salt) |
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MBS6062761-1g | MyBiosource | 1(g | EUR 460 |
Cefuroxime Sodium Salt ((6R, 7R)-3-[[(Aminocarbonyl)oxy]methyl]-7-[[(2Z)-2-furanyl(methoxyimino)acetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid Sodium Salt) |
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MBS6062761-5x1g | MyBiosource | 5x1g | EUR 1925 |
Cefuroxime |
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C248088 | Toronto Research Chemicals | 100mg | EUR 487 |
Description: 55268-75-2 |
Cefuroxime |
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GA9610 | Glentham Life Sciences | 100mg | EUR 114.58 |
Cefuroxime |
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GA9610-1 | Glentham Life Sciences | 1 | EUR 125.1 |
Cefuroxime |
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GA9610-100 | Glentham Life Sciences | 100 | EUR 39.4 |
Cefuroxime |
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GA9610-100MG | Glentham Life Sciences | 100 mg | EUR 84 |
Cefuroxime |
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GA9610-1G | Glentham Life Sciences | 1 g | EUR 187.2 |
Cefuroxime |
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GA9610-250 | Glentham Life Sciences | 250 | EUR 63.3 |
Cefuroxime |
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GA9610-250MG | Glentham Life Sciences | 250 mg | EUR 112.8 |
Cefuroxime-d3 |
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C248052 | Toronto Research Chemicals | 10mg | EUR 293 |
Cefuroxime-d3 |
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MBS6023901-1mg | MyBiosource | 1(mg | EUR 605 |
Cefuroxime-d3 |
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MBS6023901-5x1mg | MyBiosource | 5x1mg | EUR 2565 |
Cefuroxime axetil |
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584893 | MedKoo Biosciences | 350.0mg | EUR 535 |
Cefuroxime Axetil |
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C248065 | Toronto Research Chemicals | 100mg | EUR 227 |
Description: 64544-07-6 |
Cefuroxime axetil |
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C6478-25 | ApexBio | 25mg | EUR 45 |
Cefuroxime axetil |
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T1227-10mg | TargetMol Chemicals | 10mg | Ask for price |
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Cefuroxime axetil |
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Cefuroxime axetil |
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Cefuroxime axetil |
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Description: Cefuroxime axetil |
Cefuroxime axetil |
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Description: Cefuroxime axetil |
Cefuroxime Axetil |
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MBS6094618-100mg | MyBiosource | 100(mg | EUR 610 |
Cefuroxime Axetil |
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MBS6094618-5x100mg | MyBiosource | 5x100mg | EUR 2600 |
Cefuroxime axetil |
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MBS3842329-10mg | MyBiosource | 10mg | EUR 170 |
Cefuroxime axetil |
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MBS3842329-50mg | MyBiosource | 50mg | EUR 310 |
Cefuroxime axetil |
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MBS3842329-5mg | MyBiosource | 5mg | EUR 140 |
Cefuroxime axetil |
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MBS3842329-5x50mg | MyBiosource | 5x50mg | EUR 1380 |
Descarbamoyl Cefuroxime |
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D288830 | Toronto Research Chemicals | 10mg | EUR 198 |
Description: 56271-94-4 |
Descarbamoyl cefuroxime |
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T11000-10mg | TargetMol Chemicals | 10mg | Ask for price |
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Descarbamoyl cefuroxime |
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Descarbamoyl cefuroxime |
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Descarbamoyl cefuroxime |
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Descarbamoyl cefuroxime |
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Descarbamoyl Cefuroxime |
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MBS6076803-5mg | MyBiosource | 5(mg | EUR 525 |
Descarbamoyl Cefuroxime |
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MBS6076803-5x5mg | MyBiosource | 5x5(mg | EUR 2210 |
Descarbamoyl cefuroxime |
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MBS5766477-5mg | MyBiosource | 5mg | EUR 915 |
Descarbamoyl cefuroxime |
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MBS5766477-5x5mg | MyBiosource | 5x5mg | EUR 3970 |
Cefuroxime (free base) |
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412546 | MedKoo Biosciences | 100.0mg | EUR 418 |
Cefuroxime free acid |
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C282-1G | TOKU-E | 1 g | EUR 79.57 |
Description: C16H16N4O8S |
Cefuroxime free acid |
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C282-5G | TOKU-E | 5 g | EUR 264.35 |
Description: C16H16N4O8S |
Cefuroxime Axetil-d3 |
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C248067 | Toronto Research Chemicals | 25mg | EUR 11200 |
Cefuroxime CXM 30 mcg |
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SD061-1PK | EWC Diagnostics | 1 unit | EUR 6.31 |
Description: Cefuroxime CXM 30 mcg |
Cefuroxime CXM 30 mcg |
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SD061-1VL | EWC Diagnostics | 1 unit | EUR 2.99 |
Description: Cefuroxime CXM 30 mcg |
Cefuroxime CXM 30 mcg |
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SD061-5CT | EWC Diagnostics | 1 unit | EUR 6.65 |
Description: Cefuroxime CXM 30 mcg |
Cefuroxime CXM 30 mcg |
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SD061-5VL | EWC Diagnostics | 1 unit | EUR 11.84 |
Description: Cefuroxime CXM 30 mcg |
Cefuroxime CXM 30 mcg |
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SD061-5X50DS | EWC Diagnostics | 1 unit | EUR 6.5 |
Description: Cefuroxime CXM 30 mcg |
Di(cefuroxime Ethyl) Ether |
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D431300 | Toronto Research Chemicals | 5mg | EUR 800 |
Description: 1202925-10-7 |
(E)-Cefuroxime-d3 Axetil |
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C248077 | Toronto Research Chemicals | 100mg | EUR 17000 |
Descarbamoyl Cefuroxime-d3 |
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D288832 | Toronto Research Chemicals | 10mg | EUR 224 |
Descarbamoyl Cefuroxime-d3 |
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MBS6028446-1mg | MyBiosource | 1(mg | EUR 605 |
Descarbamoyl Cefuroxime-d3 |
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MBS6028446-5x1mg | MyBiosource | 5x1mg | EUR 2565 |
Δ2-Cefuroxime Methyl Ester |
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C248085 | Toronto Research Chemicals | 50mg | EUR 173 |
Cefuroxime Axetil Impurity C (85%) |
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C248080 | Toronto Research Chemicals | 10mg | EUR 775 |
Description: 76598-06-6 |
Cefuroxime Axetil (USP grade powder) |
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51R-U098220 | Fitzgerald | 350 mg | EUR 580 |
Description: Cefuroxime Axetil (USP grade powder) chemical reference substance |
Cefuroxime Axetil (USP grade powder) |
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MBS539936-350mg | MyBiosource | 350mg | EUR 950 |
Cefuroxime Axetil (USP grade powder) |
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MBS539936-5x350mg | MyBiosource | 5x350mg | EUR 4120 |
Desacetyloxyethyl (E)-Cefuroxime Axetil |
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D289280 | Toronto Research Chemicals | 10mg | EUR 295 |
Description: 97232-97-8 |
Cefuroxime Ezy MIC Strip CXM (0.016-256m |
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EM102-10ST | EWC Diagnostics | 1 unit | EUR 21.95 |
Description: Cefuroxime Ezy MIC Strip CXM (0.016-256m |
Cefuroxime Ezy MIC Strip CXM (0.016-256m |
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EM102-120ST | EWC Diagnostics | 1 unit | EUR 143.06 |
Description: Cefuroxime Ezy MIC Strip CXM (0.016-256m |
Cefuroxime Ezy MIC Strip CXM (0.016-256m |
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EM102-150ST | EWC Diagnostics | 1 unit | EUR 164.38 |
Description: Cefuroxime Ezy MIC Strip CXM (0.016-256m |
Cefuroxime Ezy MIC Strip CXM (0.016-256m |
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EM102-30ST | EWC Diagnostics | 1 unit | EUR 53.07 |
Description: Cefuroxime Ezy MIC Strip CXM (0.016-256m |
Cefuroxime Ezy MIC Strip CXM (0.016-256m |
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EM102-60ST | EWC Diagnostics | 1 unit | EUR 95.53 |
Description: Cefuroxime Ezy MIC Strip CXM (0.016-256m |
Cefuroxime Ezy MIC Strip CXM (0.016-256m |
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EM102-90ST | EWC Diagnostics | 1 unit | EUR 124.27 |
Description: Cefuroxime Ezy MIC Strip CXM (0.016-256m |
Delta2-Cefuroxime Axetil (Cefuroxime Axetil Delta-3 Isomer) |
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C248070 | Toronto Research Chemicals | 10mg | EUR 2325 |
Description: 123458-61-7 |
5-Aminomethyl-2-carboxy Cefuroxime |
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C249000 | Toronto Research Chemicals | 25mg | EUR 9200 |
Cefuroxime Axetil d-3 iso (USP grade powder) |
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51R-U098231 | Fitzgerald | 10 mg | EUR 1761 |
Description: Cefuroxime Axetil d-3 iso (USP grade powder) chemical reference substance |
Cefuroxime Axetil d-3 iso (USP grade powder) |
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MBS539937-10mg | MyBiosource | 10mg | EUR 2820 |
Cefuroxime Axetil d-3 iso (USP grade powder) |
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MBS539937-5x10mg | MyBiosource | 5x10mg | EUR 12550 |
Cefuracetime (E-isomer) Sodium Salt |
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C243940 | Toronto Research Chemicals | 100mg | EUR 234 |
Description: 97170-20-2 |
Biochemical Test Kit for Salmonella |
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K141-10KT | EWC Diagnostics | 1 unit | EUR 25.63 |
Description: Biochemical Test Kit for Salmonella |
Biochemical Test Kit for Salmonella |
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K141-5KT | EWC Diagnostics | 1 unit | EUR 14.23 |
Description: Biochemical Test Kit for Salmonella |
HiMotility™ Biochemical Kit for Salmonel |
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KBM002-5KT | EWC Diagnostics | 1 unit | EUR 29.67 |
Description: HiMotility™ Biochemical Kit for Salmonel |
Biochemical Test Kit for Shigella |
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K140-10KT | EWC Diagnostics | 1 unit | EUR 130.52 |
Description: Biochemical Test Kit for Shigella |
Biochemical Test Kit for Shigella |
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K140-5KT | EWC Diagnostics | 1 unit | EUR 72.53 |
Description: Biochemical Test Kit for Shigella |
HiMotility™ Biochemical Kit for |
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KBM002-10KT | EWC Diagnostics | 1 unit | EUR 50.53 |
Description: HiMotility™ Biochemical Kit for |
HiMotility™ Biochemical Kit for |
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KBM002-20KT | EWC Diagnostics | 1 unit | EUR 72.14 |
Description: HiMotility™ Biochemical Kit for |
HiMotility™ Biochemical Kit for E.coli |
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KBM001-10KT | EWC Diagnostics | 1 unit | EUR 50.53 |
Description: HiMotility™ Biochemical Kit for E.coli |
HiMotility™ Biochemical Kit for E.coli |
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KBM001-20KT | EWC Diagnostics | 1 unit | EUR 72.14 |
Description: HiMotility™ Biochemical Kit for E.coli |
Biochemical Test Kit for Vibrio cholera |
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K138-10KT | EWC Diagnostics | 1 unit | EUR 94.6 |
Description: Biochemical Test Kit for Vibrio cholera |
Biochemical Test Kit for Vibrio cholera |
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K138-5KT | EWC Diagnostics | 1 unit | EUR 49.85 |
Description: Biochemical Test Kit for Vibrio cholera |
HiMotility™ Biochemical Kit for V. chol |
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KBM004-10KT | EWC Diagnostics | 1 unit | EUR 68.78 |
Description: HiMotility™ Biochemical Kit for V. chol |
HiMotility™ Biochemical Kit for V. chol |
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KBM004-20KT | EWC Diagnostics | 1 unit | EUR 98.33 |
Description: HiMotility™ Biochemical Kit for V. chol |
HiMotility™ Biochemical Kit for V. chol |
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KBM004-5KT | EWC Diagnostics | 1 unit | EUR 40.38 |
Description: HiMotility™ Biochemical Kit for V. chol |
Biochemical Test Kit for Vibrio parahaem |
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K139-10KT | EWC Diagnostics | 1 unit | EUR 94.92 |
Description: Biochemical Test Kit for Vibrio parahaem |
Biochemical Test Kit for Vibrio parahaem |
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K139-5KT | EWC Diagnostics | 1 unit | EUR 50 |
Description: Biochemical Test Kit for Vibrio parahaem |
HiMotility™ Biochemical Kit for V. parah |
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KBM005-10KT | EWC Diagnostics | 1 unit | EUR 68.78 |
Description: HiMotility™ Biochemical Kit for V. parah |
HiMotility™ Biochemical Kit for V. parah |
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KBM005-20KT | EWC Diagnostics | 1 unit | EUR 98.33 |
Description: HiMotility™ Biochemical Kit for V. parah |
HiMotility™ Biochemical Kit for V. parah |
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KBM005-5KT | EWC Diagnostics | 1 unit | EUR 40.38 |
Description: HiMotility™ Biochemical Kit for V. parah |
HiMotility™ Biochemical Kit for Listeria |
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KBM003A-10KT | EWC Diagnostics | 1 unit | EUR 50.53 |
Description: HiMotility™ Biochemical Kit for Listeria |
HiMotility™ Biochemical Kit for Listeria |
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KBM003A-20KT | EWC Diagnostics | 1 unit | EUR 72.14 |
Description: HiMotility™ Biochemical Kit for Listeria |
HiMotility™ Biochemical Kit for Listeria |
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KBM003A-5KT | EWC Diagnostics | 1 unit | EUR 29.67 |
Description: HiMotility™ Biochemical Kit for Listeria |
HiMotility™ Biochemical Kit for Shigella |
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KBM006-10KT | EWC Diagnostics | 1 unit | EUR 68.83 |
Description: HiMotility™ Biochemical Kit for Shigella |
HiMotility™ Biochemical Kit for Shigella |
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KBM006-20KT | EWC Diagnostics | 1 unit | EUR 98.33 |
Description: HiMotility™ Biochemical Kit for Shigella |