Transcription and Enhancers

Abstract Active transcriptional enhancers are often transcribed into eRNA, the changing levels of which mirror those of the mRNA of the target gene. We discuss some of the reported functions of these RNAs and their likely diversity to allow the utilization of distinct cis-regulatory regions to enhance transcription in various…

Thymine Dimer

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Lac Operon Induction in Escherichia coli

Abstract The most widely used expression systems in bacteria are based on the Escherichia coli lac promoter. Furthermore, elements of the lac operon are used today in systems and synthetic biology. In most cases, the free inducers IPTG or TMG are used. Here we report a systematic comparison of lac…